The Gadsden flag, offering a rattlesnake and the phrase "Don't Tread on Me

https://manuelasvb291.keystonescope.com/posts/collecting-civil-war-flags-a-guide-for-enthusiasts-3

The Gadsden flag, featuring a rattlesnake and the phrase "Don't Tread on Me," become followed with the aid of some Confederate gadgets as a symbol of resistance opposed to perceived oppression.